COMMISSION DELEGATED REGULATION (EU) 2024/1229
of 20 February 2024
supplementing Regulation (EU) 2019/4 of the European Parliament and of the Council by establishing specific maximum levels of cross-contamination of antimicrobial active substances in non-target feed and methods of analysis for these substances in feed
(Text with EEA relevance)
Article 1
Subject matter and scope
Article 2
Specific maximum levels of cross-contamination of antimicrobial active substances in non-target feed
Article 3
Methods of analysis for antimicrobial active substances in feed
Article 4
Entry into force and application
ANNEX
Maximum levels of cross-contamination in non-target feed as referred to in Article 2(2) and reference methods of analysis for the quantification of the level of cross-contamination of antimicrobial active substances in feed as referred to in Article 3
Chemical class |
Name of substance |
CAS number1 |
EU number2 |
Multi-analyte methoda b c |
Reference analytical method3 4 5 |
Maximum levels of cross-contamination in non-target feed referred to in Article 2(2) (set at the limit of quantification (LOQ)) (μg/kg) |
Polymixins (polypeptide antibiotics) |
Colistin |
1264-72-8 |
— |
(b) |
LSE – A – C – SPE – E – LC-MS/MS |
150 (Colistin A) 300 (Colistin B) |
Pyrimidine inhibitor of dihydrofolate reductase |
Trimethoprim |
738-70-5 |
212-006-2 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
25 |
Macrolides |
Tylvalosin |
63409-12-1 |
— |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |
Tilmicosin |
108050-54-0 |
639-676-2 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |
|
Tylosin |
1401-69-0 |
215-754-8 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |
|
Lincosamides |
Lincomycin |
154-21-2 |
205-824-6 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
25 |
Pleuromutilins |
Tiamulin |
55297-96-6 |
259-580-0 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
10 |
Valnemulin |
101312-92-9 |
— |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
50 |
|
Penicillins |
Amoxicillin |
26787-78-0 |
612-127-4 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
150 |
Penicillin V |
1098-87-9 |
— |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
50 |
|
Aminoglycosides |
Apramycin |
65710-07-8 |
265-890-7 253-460-1 |
(a) |
LSE – A – C – SPE – LC-MS/MS |
50 |
Neomycin |
1404-04-2 |
1404-04-2 |
(a) |
LSE – A – C – SPE – LC-MS/MS |
50 |
|
Paromomycin |
1263-89-4 |
— |
(a) |
LSE – A – C – SPE – LC-MS/MS |
50 |
|
Spectinomycin |
1695-77-8 |
— |
(a) |
LSE – A – C – SPE – LC-MS/MS |
500 |
|
Amphenicols |
Florfenicol |
73231-34-2 |
642-986-0 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
150 |
Tiamfenicol |
15318-45-3 |
239-355-3 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
200 |
|
Coccidiostats |
Amprolium |
137-88-2 |
204-458-4 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |
|
|
|
(a) |
LSE – A – C – SPE – LC-MS/MS |
5 |
|
Fluoroquinolones |
Flumequine |
42835-25-6 |
255-962-6 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
25 |
Oxolinic Acid |
14698-29-4 |
238-750-8 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
25 |
|
Sulphonamides |
Sulfamonomethoxine |
1220-83-3 |
624-483-8 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
25 |
Sulfadimethoxine |
122-11-2 |
204-523-7 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
25 |
|
Tetracyclines |
Chlortetracycline |
57-62-5 |
200-341-7 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |
Doxycycline |
564-25-0 |
209-271-1 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |
|
Tetracycline |
60-54-8 |
200-481-9 |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |
|
Oxytetracycline |
79-57-2 |
— |
(c) |
LSE – US – A – C – F – LC-MS/MS or LC-HRMS |
100 |